Rational design of a novel propeptide for improving active production of Streptomyces griseus trypsin in Pichia pastoris.
نویسندگان
چکیده
Applying in silico simulations and in vitro experiments, the amino acid proline was proved to have a profound influence on Streptomyces griseus trypsinogen, and the hydrogen bond between H(57) and D(102) was found to be crucial for trypsin activity. By introducing an artificial propeptide, IVEF, the titer of trypsin was increased 6.71-fold.
منابع مشابه
Rational design of a novel propeptide for improving active
39 Applying in silico simulations and in vitro experiments, the amino acid proline was proved to 40 have a profound influence on Streptomyces griseus trypsinogen, and the hydrogen bond 41 between H and D was crucial for trypsin activity. By introducing an artificial propeptide 42 IVEF, the titer of trypsin was increased by 6.71-fold. 43
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 79 12 شماره
صفحات -
تاریخ انتشار 2013